Journal: bioRxiv
Article Title: Dopamine selectively regulates pediatric sclera/choroid interactions through the stimulation of exosome-associated retinoic acid
doi: 10.1101/2025.07.13.664562
Figure Lengend Snippet: (A) Retinoic acid concentration in the separated media and exosome fractions of CCM and Dopamine-CCM. Shown is RA concentration (pM) +/- SEM, n=3. Exosomal concentration was adjusted for full CCM volume. (B) Paediatric scleral fibroblast (HSF9-posterior) collagen gel contraction in serum containing medium (10% serum) with/without RA (1µM), RAR inhibitor AGN193109 (1µM) or both.Shown is mean contraction (%) +/- SEM, n=3. (C) CCM and dopamine-CCM were prepared from paediatric choroid cells and used to stimulate paediatric scleral fibroblast (HSF9-posterior) collagen gel contraction in the presence of RA (1µM), RAR inhibitor AGN193109 (1µM) or both. Shown is mean contraction (%) +/- SEM, n=3. (D) Paediatric choroid cells (Choro9L) were treated with 10nM dopamine with/without 8µM WIN18446 for 24hrs, after which the medium was replaced with the serum-free medium for 24 hours to generate CCM and Dopamine-CCM. These were used to stimulate scleral fibroblasts (HSF9-Posterior) contraction. Shown as mean fold change in contraction (compared to serum free medium) +/- SEM (n=4). * P<0.05, ** P<0.01, (One- or Two-way way ANOVA and Tukey’s multiple comparisons tests).
Article Snippet: Human Retinoic Acid ELISA Kit (Biorbyt, UK, Cat# orb407080) was used to measure RA concentration following the manufacturer’s protocol.
Techniques: Concentration Assay